9367 tau rabbit polyclonal k9ja icc  (Cell Signaling Technology Inc)

Journal: Acta Neuropathologica

Article Title: Identification of high-performing antibodies for the reliable detection of Tau proteoforms by Western blotting and immunohistochemistry

doi: 10.1007/s00401-024-02729-7

Figure Lengend Snippet: Representative antibody validation results for WB. a–e WB data illustrating the performance of different Tau antibodies: Tau-12 Millipore #MAB2241 ( a ), K9JA Dako #A0024 ( b ), Tau-46 SCBT #sc-32274 ( c ), Tau-5 Abcam #ab80579 ( d ), AT270 (pThr181) ThermoFisher Scientific #MN1050 ( e ). For each antibody, overall performance was categorised based on a traffic light system, as defined in Figs. – . Number of citations for each antibody clone as of 9 Oct 2023 is indicated. WBs shown in columns I to V are as follows: WB of lysates from HEK293T cells overexpressing 0N3R human Tau and corresponding control cells ( column I ); WB of recombinant human Tau ladder (5 ng/isoform/lane), plus adult mouse brain lysates from wildtype, Mapt −/− and hTau mice ( column II ); WB of lysates from SH-SY5Y neuroblastoma cells, plus HAP1 cells: parental (wildtype) and two cell lines carrying either a 14 bp deletion (14 bp Δ) or a 2-bp deletion (2 bp Δ) in MAPT exon 4 ( column III ); WB of recombinant human Tau ladder (50 ng/isoform/lane) plus recombinant 2N4R Tau that has been phosphorylated by one of three known Tau kinases: GSK3ꞵ, DYRK1A or CAMKIIA ( column IV ); WB of lysates from SH-SY5Y neuroblastoma cells that have been either untreated (-) or treated ( +) with λPP. Where applicable, quantifications of the Tau signal intensity for each lane are shown superimposed as a bar chart, with the respective values [a.u.] printed on or above each bar of the chart ( column V )

Article Snippet: This was observed particularly with the K9JA (Dako, cat. no. A0024) polyclonal antibody in both mouse and human samples (Supp.

Techniques: Recombinant

Journal: Acta Neuropathologica

Article Title: Identification of high-performing antibodies for the reliable detection of Tau proteoforms by Western blotting and immunohistochemistry

doi: 10.1007/s00401-024-02729-7

Figure Lengend Snippet: Representative antibody validation results for WB. a–e WB data illustrating the performance of different Tau antibodies: Tau-12 Millipore #MAB2241 ( a ), K9JA Dako #A0024 ( b ), Tau-46 SCBT #sc-32274 ( c ), Tau-5 Abcam #ab80579 ( d ), AT270 (pThr181) ThermoFisher Scientific #MN1050 ( e ). For each antibody, overall performance was categorised based on a traffic light system, as defined in Figs. – . Number of citations for each antibody clone as of 9 Oct 2023 is indicated. WBs shown in columns I to V are as follows: WB of lysates from HEK293T cells overexpressing 0N3R human Tau and corresponding control cells ( column I ); WB of recombinant human Tau ladder (5 ng/isoform/lane), plus adult mouse brain lysates from wildtype, Mapt −/− and hTau mice ( column II ); WB of lysates from SH-SY5Y neuroblastoma cells, plus HAP1 cells: parental (wildtype) and two cell lines carrying either a 14 bp deletion (14 bp Δ) or a 2-bp deletion (2 bp Δ) in MAPT exon 4 ( column III ); WB of recombinant human Tau ladder (50 ng/isoform/lane) plus recombinant 2N4R Tau that has been phosphorylated by one of three known Tau kinases: GSK3ꞵ, DYRK1A or CAMKIIA ( column IV ); WB of lysates from SH-SY5Y neuroblastoma cells that have been either untreated (-) or treated ( +) with λPP. Where applicable, quantifications of the Tau signal intensity for each lane are shown superimposed as a bar chart, with the respective values [a.u.] printed on or above each bar of the chart ( column V )

Article Snippet: Indeed, non-selective cross-reactivity with proteins other than Tau was observed for three of the four most highly-cited “total” Tau antibodies, namely HT7 (436 citations), K9JA polyclonal Tau antibody from Dako (354 citations), and Tau-46 (300 citations) [ ].

Techniques: Recombinant